Examining Genetic Variants with Liquid Biopsy and Tumor Samples
Ocean Ridge Biosciences offers cost-effective ultra-deep sequencing of targeted genes to analyze genetic variants in solid tumors, cell-free DNA (cfDNA), and genomic DNA. Utilizing highly parallel multiplex next-generation sequencing of PCR amplicons, ORB's oncogene panel, or oncopanel, sequencing and analysis enables investigation of cancer-related genes or genomic regions, such as tumor suppressor genes such as BRCA1 or TP53, and oncogenes that can turn healthy cells into cancer cells. The focused nature of oncopanels can reduce costs and increase the speed of genomic studies because only a limited set of genes are interrogated. Additional benefits include advanced detection of low-frequency variants with deeper sequencing options and production of more manageable datasets than traditional whole genome sequencing studies.
Sample Types and Sample Submission Instructions
- Tumor biopsies
- Genomic DNA (gDNA)
- Cell-free DNA (cfDNA)
- Contact us about other sample types
|ORB provides cfDNA extraction services with blood-based biofluid samples and genomic DNA isolation from tumor tissues. ORB is a Biosafety Level 2 (BSL-2) laboratory, and can accept samples that contain agents associated with human diseases.|
ORB provides cfDNA extraction services with blood-based biofluid samples and genomic DNA isolation from tumor tissues. ORB is a Biosafety Level 2 (BSL-2) laboratory, and can accept samples that contain agents associated with human diseases.
Common Oncology Areas Utilizing Oncopanels
- Breast cancer
- Colorectal cancer
- Pancreatic cancer
- Ovarian cancer
- Prostate cancer
- Non-small cell lung cancer
- Renal cell carcinoma
ORB’s Oncogene Analysis Service
ORB offers analysis of numerous genetic variant types using oncopanels from industry leading manufacturers where starting materials are optimized for low-input and degraded samples, e.g. FFPE tissues. Many panels require as little as 10 ng of gDNA per sample, please see Table I for a list of available panels and associated variant types. Contact us to determine which panel would be most appropriate for your cancer investigation.
Table I. Oncopanels available for analysis using ORB's oncogene analysis service.
|Manufacturer||Panel||Genes||Cancer Type||FFPE||Variant Types|
|Germline||Somatic||INDELS||SNPs||Gene Fusions||Structural||Copy Number||Loss of Heterozygosity||Chromosomal Abnormalities||Short Tandem Repeats|
|Illumina||TruSight Cancer Sequencing||94||Pan-Cancer||Yes|
|Illumina||TruSight Tumor 15||15||Solid Tumor||Yes||Yes||Yes|
|Illumina||TruSight Myeloid Sequencing||Up tp 54||Hematological||Yes||Yes|
|Illumina||TruSeq Amplicon – Cancer||48||Pan-Cancer||Yes||Yes||Yes|
|Illumina||TruSight RNA Pan-Cancer||1385||Pan-Cancer||Yes|
|Illumina||TruSight Tumor 170||170||Solid Tumor||Yes||Yes||Yes||Yes||Yes||Yes||Yes|
|Illumina||TruSight RNA Fusion||507 gene fusion events||Pan-Cancer||Yes||Yes|
|Illumina||TruSight Custom Amplicon Low Input||Up to 1536 amplicons||Pan-Cancer||Yes||Yes||Yes||Yes||Yes||Yes||Yes||Yes||Yes||Yes|
|Swift Biosciences||Accel-Amplicon 56G Oncology Panel v2||56||Yes||Yes||Yes||Yes|
|Swift Biosciences||Accel-Amplicon BRCA1 and BRCA2||2||Yes||Yes||Yes||Yes|
|Swift Biosciences||Accel-Amplicon BRCA1, BRCA2, and PALB2||3||Yes||Yes||Yes||Yes|
|Swift Biosciences||Accel-Amplicon Comprehensive TP53||1||Yes||Yes||Yes||Yes|
|Swift Biosciences||Accel-Amplicon EGFR Pathway||4||Yes||Yes||Yes||Yes|
Contact Us to learn how ORB’s oncopanel service can facilitate a deep view of the genetics in your oncology research!